Nikon A1R Confocal Laser Scanning Inverted Microscope

Description

The system is based around fully motorized Nikon Eclipse Ti body with focus correction hardware. The microscope is located in an incubation cage for live imaging when a control over CO2 concentration, temperature and humidity is required. Equipped with Nikon DS-Qi1Mc camera for wide-field image acquisition.

Lasers/ Channels of Confocal

4 visible solid state (Diode) lasers:

406 nm (violet, 50mW) for Blue channel (emission filter: 425-475nm)

492 nm (blue, 50mW) for Green channel (emission filter: 500-550nm)

561nm (green, 10mW) for Red channel (emission filter: 570-620nm)

639 nm (red, 40mW) for Far-Red channel (emission filter: 662.5-737.5nm)

Objectives

Plan Fluor 10x DIC L; NA:

Plan Apochromat VC 20x DIC N2; NA:

Plan Fluor 40x Oil DIC H N2; NA:

Plan Apochromat VC 60x Oil DIC N2; NA:

Scanners

Galvano scanner for standard image acquisition, up to 4096 x 4096 px

Resonant scanner for high speed image acquisition, up to 512 x 512 px

Detectors

Detector unit – 4 Photo Multiplier Tubes (PMTs)

Spectral detector – 32 channels, 400-750 nm, up to 2048 x 2048 px, unmixing

Diascopic PMT detector

Wide-field Channels

                      Excitation filter            Emission filter                    

Blue                 340-380 nm                  435-485 nm                  

Green              465-495 nm                  515-555 nm                   

Red                  528-553 nm                  590-650 nm                   

Analyzer (DIC) – Halogen lamp for transmitted illumination       

Bright Field (no filters) – Halogen lamp for transmitted illumination       

Camera for Wide-field and Bright-field Imaging

Nikon DS-Qi1Mc (Monochrome) – 1.5 Mpx

Software

2D analysis, 2D volume rendering/orthogonal, 4D analysis, spectral anmixing, FRAP, FLIP, FRET, photoactivation, 3D/multipoint time-lapse imaging, and more.