Nikon Eclipse Ti2-E Inverted Wide-field Fluorescence Microscope

Description

The system is based around fully motorized Nikon Eclipse Ti2-E body. Equipped with on-stage removable incubation chamber for live imaging when a control over CO2 concentration, temperature and humidity is required.

Channels

                     Excitation filter        Emission filter                    

Blue                 340-380 nm                 435-485 nm                  

Green               465-495 nm                515-555 nm                   

Red                   528-553 nm                590-650 nm                   

Far-red             625-650 nm                Peak 670 nm                

Analyzer (DIC) – High power LED for Transmitted illumination       

Bright Field (no filters) – High power LED for Transmitted illumination       

Light Source for Epi Fluorescence

SOLA Light Engine – a solid-state illumination that provides white light output for excitation of DAPI, GFP/FITC, YFP, Cy3, mCherry, Cy5 and spectrally similar fluorophores.

Objectives

CFI Plan Fluor 4x; NA: 013; WD: 17 mm

CFI Plan Fluor 10x DIC L/N1; NA: 0.3; WD: 16 mm

CFI S Plan Fluor ELWD 20x DIC N2; NA: 0.45; WD: 8.2-6.9 mm; correction: 0-2 mm

CFI S Plan Fluor ELWD 40x DIC N2; NA: 0.6; WD: 3.6-2.8 mm; correction: 0-2 mm

CFI Plan Apochromat Lambda 60x Oil DIC N2; NA: 1.4; WD: 0.13 mm

CFI Plan Apochromat Lambda 100x Oil DIC N2; NA: 1.45; WD: 0.13 mm

Cameras

Nikon DS-Fi3 5.9 Mpx color camera

Andor Zyla 4.2 fast and sensitive sCMOS camera

Software

NIS-Elements